home - Research - Current Research
 

Notice Board Topics

Seminar Series There are currently no seminars scheduled.…
Follow us on Twitter http://twitter.com/PainConsortium
News, Videos and Features News

Chronic pain: the search for a killer

Researchers engaging with local communities

Researchersí discovery of why sunburn hurts reveals possible target for new pain relief drugs

New award funding of LPC

UK researchers tackle pain

Imperial College and the London Pain Consortium partner with a Japanese chemical company to fight chronic pain

Videos

Walking on Fire click here

Features

London Pain Consortium: key Discoveries

Reserachers: Pain killers click here

Painstaking research - tackling chronic pain click here

The London Pain Consortium making a difference
 
Current Research
Protein expression profiling of laser microdissected-dorsal horn laminae of chronic pain model rats.
Many aspects of chronic pain involve related events at dorsal horn synapses, namely synaptic plasticity in spinal dorsal horn neurons. We combine laser scanning microdissector, 1D/2D SDS-PAGE, and mass spectrometry to identify changes in protein expressions in chronic pain models. Specific laminae of contra- and ipsilateral dorsal horn of chronic pain model rats are isolated by a laser microdissector. Proteins in the isolated laminae are analysed by 1/2D SDS-PAGE and subsequent MALDI-TOF and ESI Q-TOF MS/MS mass spectrometry. In this way we can identify the changes of expression levels of molecules not only in the postsynaptic cells, but also in the presynaptic terminals of primary sensory neurons which cannot be studied by genetic approaches such as microarrays. The changes of expression levels of proteins are further examined by immunohistochemistry. Potential functional consequences of altered protein expression will be examined in neuronal tissue culture using over-expression and siRNA approaches prior to in vivo studies.