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Chronic pain: the search for a killer

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New award funding of LPC

UK researchers tackle pain

Imperial College and the London Pain Consortium partner with a Japanese chemical company to fight chronic pain


Walking on Fire click here


London Pain Consortium: key Discoveries

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Painstaking research - tackling chronic pain click here

The London Pain Consortium making a difference
Wellcome Trust conference The Challenges of Chronic Pain

11-13 March 2015
Wellcome Trust Genome Campus, Hinxton, Cambridge, UK

Abstract deadline: 30 January 2015
Registration deadline: 16 February 2015

For more information
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Current Research
Protein expression profiling of laser microdissected-dorsal horn laminae of chronic pain model rats.
Many aspects of chronic pain involve related events at dorsal horn synapses, namely synaptic plasticity in spinal dorsal horn neurons. We combine laser scanning microdissector, 1D/2D SDS-PAGE, and mass spectrometry to identify changes in protein expressions in chronic pain models. Specific laminae of contra- and ipsilateral dorsal horn of chronic pain model rats are isolated by a laser microdissector. Proteins in the isolated laminae are analysed by 1/2D SDS-PAGE and subsequent MALDI-TOF and ESI Q-TOF MS/MS mass spectrometry. In this way we can identify the changes of expression levels of molecules not only in the postsynaptic cells, but also in the presynaptic terminals of primary sensory neurons which cannot be studied by genetic approaches such as microarrays. The changes of expression levels of proteins are further examined by immunohistochemistry. Potential functional consequences of altered protein expression will be examined in neuronal tissue culture using over-expression and siRNA approaches prior to in vivo studies.